Full time
Harvard University
Boston, MA
The Sharpe laboratory at Harvard Medical School seeks a highly motivated individual to support research in mouse and human tumor immunology, and/or autoimmunity. Under the supervision of the Lab Manager and/or senior trainees, this individual will perform a wide range of highly sophisticated laboratory techniques, working closely with fellows and students to analyze the functions of immunoregulatory pathways. This includes immunological techniques (immune cell isolation, assays of stromal, myeloid, and T cell function, cytokine ELISA assays, multiparameter flow cytometry, handling recombinant DNA vectors), molecular biology procedures (PCR, RNA and/or DNA preparation from tissues, as well as bulk and single-cell RNASeq). Animal work is required (measuring tumors, monitoring disease scores, weighing mice, survival bleeding, i.p. / s.c. / i.v. injections, generating chimeras), mouse husbandry (colony maintenance, genotyping). This individual may also handle primary human samples...